On the cooperative and noncooperative binding of ethidium to DNA.
نویسندگان
چکیده
The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other hand, no evidence of cooperative binding was observed in the binding isotherms obtained with calf thymus, C. perfringens, M. lysodeikticus, or poly(dG-dC) X (dG-dC) under the experimental conditions used (0.1 M NaCl).
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 10 24 شماره
صفحات -
تاریخ انتشار 1982